Protein_Domain
Strep-Tag
Part:BBa_I757014:Design
Designed by: iGEM Team Freiburg 2007 Group: iGEM07_Freiburg (2007-10-24)
Strep-Tag II affinity tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
between BioBrick 1.0 sites part is flanked 5' by NgoMIV and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions
Source
synthetic DNA by GeneArt
References
Not a comprehensive list
<biblio>
- Schmidt93 pmid=8433964
// this looks to be the original reference for the Strep-tag
- Skerra96 pmid=8636976
// original reference for Strep-tag II
- Voss97 pmid=9415448
// original reference for streptavidin mutant with enhanced affinity for Strep-tag and Strep-tag II. The variant was later called StrepTactin.
- Skerra99 pmid=10796988
- Skerra00 pmid=11036648
// history of Strep-tag system
- Skerra07 pmid=17571060
// includes brief overview of Strep-tag system </biblio>